New lipase-producing Streptomyces isolated from halo-alkaline habitat in Wadi El Natrun: polyphasic identification and statistical optimization of enzyme production

Abstract Background Bioprospecting lipase producers in non-conventional habitats are the way to find special enzymes of diverse applications. Halo-alkaline marshes Wadi El Natrun Egypt some most stable ecological systems world, and because double extremities alkalinity salinity, they harbor individual microbes capable adapting stress conditions. Results Eight strains were recovered from coastline soil Al-Beida Lake have been tested for production. Among eight isolates, strain SBLWN_MH2 was active producer (7.5 U/ml). The crude showed activity over a wide pH range (3.5 13) with an optimum at 10.5, it able show more than 75% its highest elevated up 13. identification using phenotypic genotypic methods strongly indicated that belonged genus Streptomyces similarity 99%. Thus, has given suggested name sp. (MG593538). produced extracellular modified starch casein medium supplemented different oils or Tween-80, potential production rate attained case linseed oil after 3 days. Further experiments carried out optimize composition through Box-Behnken design response surface methodology, possible achieve 3.5-fold increase Conclusions present study indicates is could be fruitfully employed large-scale highly alkaline lipase.